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Now, the test itself involves taking a sample of the tumor (usually from a biopsy) and treating it with special fluorescent probes. These probes are designed to attach to the HER2 gene. Under a special microscope, doctors can see how many copies of the HER2 gene are present within the cancer cells. This tells them whether the cancer is **HER2-positive**, **HER2-negative**, or something in between. Getting this information right is essential because it *completely* changes how doctors approach treatment. In the context of the **iHER2 FISH negative meaning**, it indicates that the cancer cells do not have an excess amount of the HER2 gene.
